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| NISHIKAWA, Yoshifumi; IBRAHIM, Hany M.; KAMEYAMA, Kyohko; SHIGA, Ikumi; HIASA, Jun; XUAN, Xuenan; 西川, 義文; 玄, 学南. |
| The intracellular protozoan Toxoplasma gondii lacks the ability to synthesize sterol and scavenges cholesterol from the lowdensity lipoprotein receptor (LDLR) pathway of its host to facilitate replication. Sterol biosynthesis inhibitors, however, have a demonstrated anti-Toxoplasma effect. In this study, we examined the host mevalonate pathway as a novel source of cholesterol for T. gondii and its effects on parasite growth in macrophages. Parasite growth did not significantly change in the absence of LDLR or when LDL was exogenously supplemented. Lovastatin and compactin, both inhibitors of hydroxymethylglutaryl-CoA (HMG-CoA) reductase in the mevalonate pathway, significantly inhibited T. gondii growth in both wild-type and LDLR-knockout macrophages.... |
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Palavras-chave: Cholesterol; Lipid metabolism; Macrophage; Parasitology; Toxoplasma gondii. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3583 |
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| NISHIMURA, Maki; TOYOTA, Yumi; ISHIDA, Yoshikazu; NAKAYA, Hideki; KAMEYAMA, Kyohko; NISHIKAWA, Yoshifumi; MIYAHARA, Kazuro; INOKUMA, Hisashi; FURUOKA, Hidefumi; 西川, 義文; 猪熊, 壽; 古岡, 秀文. |
| A 9-month-old steer was autopsied due to recurrent ruminal tympany. A macroscopic examination found an enlarged caudal mediastinal lymph node, and a section of the lymph node revealed necrosis with marked calcification, similar to tuberculous lymphadenitis. Histopathologically, the lesion consisted of multiple coagulative necrotic foci and fibrosis with macrophage, lymphocyte, eosinophil and multinucleated giant cell infiltration. Non-uniform width hyphae were detected in the necrotic area and within the cytoplasm of the multinucleated giant cells, and they were found to be anti-Rhizopus arrhizus antibody positive in an immunohistochemical examination. Therefore, the steer was diagnosed with necrotic caudal mediastinal lymphadenitis due to zygomycetes... |
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Palavras-chave: Cattle; Necrotic lymphadenitis; Ruminal tympany; Zygomycosis. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3943 |
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